Journal: Nucleic Acids Research

Article Title: Breaking the stoichiometric limit of padlock probe ligation via catalytic hairpin assembly-and-cyclization

doi: 10.1093/nar/gkag277

Figure Lengend Snippet: Optimizations based on gel electrophoresis ( a – e ) and optomagnetic sensing ( f – h ). (a) CHA with varying toehold length of H1*. (b) Dose-response of CHA with varying toehold length of H2*. (c) Grayscale intensity analysis of the electrophoretic bands corresponding to products and reactants in panel (b). (d, e) CHAC-RCA mediated by T4 DNA ligase (22°C–37°C) and Ampligase (37°C–52°C) using H2 variants with varying 5′-terminal complementary region lengths. (f) Optomagnetic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $ - {\mathrm{\Delta }}\bar{\varphi }$\end{document} values for orthogonal combinations of H1 (7, 8, 9 nt of toehold) and H2 (7, 8, 9, 10 nt of toehold) variants. (g) Optomagnetic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $ - {\mathrm{\Delta }}\bar{\varphi }$\end{document} values for CHAC-RCA assays with different H2:H1 molar ratios (1 nM of H1, 37°C, 1 h each of CHAC and RCA). (h) Optomagnetic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $ - {\mathrm{\Delta }}\bar{\varphi }$\end{document} values for CHAC-RCA assays with different CHAC times (1 nM of H1, 10 nM of H2, 37°C, 1 h of RCA). Error bars indicate the standard deviation based on three independent replicates.

Article Snippet: DNA exonuclease I and III were obtained from New England BioLabs (Ipswich, MA).

Techniques: Nucleic Acid Electrophoresis, Standard Deviation

Journal: Nucleic Acids Research

Article Title: Breaking the stoichiometric limit of padlock probe ligation via catalytic hairpin assembly-and-cyclization

doi: 10.1093/nar/gkag277

Figure Lengend Snippet: Practicability validations. ( a ) Influences of background random DNA on the CHAC-RCA assay. ( b ) Detection of different sequences (10 pM) with varying mutations. ( c ) Serum concentration-dependent optomagnetic signal reduction for 10 pM of target molecule. ( d ) Optomagnetic and qPCR results for 15 clinical skin swab specimens. Error bars indicate standard deviations from three independent replicates. Horizontal dashed lines indicate the cutoff value.

Article Snippet: DNA exonuclease I and III were obtained from New England BioLabs (Ipswich, MA).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Synthesis and structural insights of tunable NiO X –MoO 3 –MoS 2 nanocomposites with enhanced photocatalytic performance

doi: 10.1038/s41598-026-36921-4

Figure Lengend Snippet: HR-TEM images and related analyses of NiO X NPs and NMOS-I-III NCs are presented as follows: ( A ) NiO X NPs, with an inset showing a high-resolution image of a single NP; ( B ) Size distribution histogram of NiO X NPs; ( C ) SAED pattern of NiO X NPs; ( D ) annealed NiO X NPs, with an inset showing a high-resolution image of a single NP; ( E ) Size distribution histogram of annealed NiO X NPs; ( F ) SAED pattern of annealed NiO X NPs; ( G , J , M ) HR-TEM images of NMOS-I-III NCs displaying particle morphology; ( H , K , N ) corresponding EDS mapping for NMOS-I-III NCs; and ( I , L , O ) images of the lattice fringes for NMOS-I-III NCs, respectively.

Article Snippet: The photoluminescence (PL) spectra of the NiO X NPs and NMOS-I-III NCs were recorded using FP-8350 Spectrofluorometer (Jasco, Japan).

Techniques:

Journal: Scientific Reports

Article Title: Synthesis and structural insights of tunable NiO X –MoO 3 –MoS 2 nanocomposites with enhanced photocatalytic performance

doi: 10.1038/s41598-026-36921-4

Figure Lengend Snippet: ( A ) Absorption spectra of NiO X NPs and NMOS-I-III NCs with various Mo-precursor ratios; ( B – E ) spectral deconvolution of NiO X and NMOS-I-III NCs.

Article Snippet: The photoluminescence (PL) spectra of the NiO X NPs and NMOS-I-III NCs were recorded using FP-8350 Spectrofluorometer (Jasco, Japan).

Techniques:

Journal: Scientific Reports

Article Title: Synthesis and structural insights of tunable NiO X –MoO 3 –MoS 2 nanocomposites with enhanced photocatalytic performance

doi: 10.1038/s41598-026-36921-4

Figure Lengend Snippet: Emission spectra of NiO NPs, NMOS-I, NMOS-II, and NMOS-III NCs measured at excitation wavelengths of 250 nm ( A ) and 532 nm ( B ). Deconvoluted PL spectra of ( C ) NiO X and ( D – F ) NMOS-I-III NCs at an excitation wavelength of 532 nm.

Article Snippet: The photoluminescence (PL) spectra of the NiO X NPs and NMOS-I-III NCs were recorded using FP-8350 Spectrofluorometer (Jasco, Japan).

Techniques:

Journal: Scientific Reports

Article Title: Synthesis and structural insights of tunable NiO X –MoO 3 –MoS 2 nanocomposites with enhanced photocatalytic performance

doi: 10.1038/s41598-026-36921-4

Figure Lengend Snippet: EPR spectra acquired under visible-light illumination of ( A ) NiO X and NMOS-I-III with BMPO as a “spin-trap” and ( B ) NMOS-II NCs under light illumination with and without the addition of DMSO.

Article Snippet: The photoluminescence (PL) spectra of the NiO X NPs and NMOS-I-III NCs were recorded using FP-8350 Spectrofluorometer (Jasco, Japan).

Techniques:

Journal: Scientific Reports

Article Title: Synthesis and structural insights of tunable NiO X –MoO 3 –MoS 2 nanocomposites with enhanced photocatalytic performance

doi: 10.1038/s41598-026-36921-4

Figure Lengend Snippet: ( A ) 3D UV-visible spectra of MB photodegradation after different light irradiation times using NiO X and NMOS-I-III NCs in aqueous solutions. Note: spectra are shown with an offset for clarity. ( B ) Schematic mechanism of methylene blue degradation by NMOS NCs.

Article Snippet: The photoluminescence (PL) spectra of the NiO X NPs and NMOS-I-III NCs were recorded using FP-8350 Spectrofluorometer (Jasco, Japan).

Techniques: Irradiation